MilliporeSigma™ anti-Siglec-1 (CD169) Clone: 5F1.1,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABT328MI-Each-of-1 | In Stock | ₹ 40,292.08 |
MABT328MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 40,292.08
GST (18%): ₹ 7,252.574
Total Price: ₹ 47,544.654
Antigen
Siglec-1 (CD169)
Classification
Monoclonal
Conjugate
Unconjugated
Gene Accession No.
Q9BZZ2
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Expected to react with all three spliced isoforms of human Siglec-1 (CD169)/Sialoadhesin reported by UniProt (Q9BZZ2).
Content And Storage
2°C to 8°C for one year from date of shipment
Isotype
IgG2a κ
Applications
Immunohistochemistry (Paraffin), Western Blot
Clone
5F1.1
Formulation
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Symbols
SIGLEC1, SN
Immunogen
GST-tagged recombinant human Siglec-1 (CD169) N-terminal fragment containing the Ig-like V-type domain.
Quantity
100 μg
Research Discipline
Cell Structure
Gene ID (Entrez)
NP_075556
Target Species
Human, Mouse
Form
Purified
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Description
- Specifically detects Siglec-1 (CD169) Clone: 5F1.1 in Human, Mouse samples, and it is validated for Immunohistochemistry (Paraffin), Western Blotting Sialoadhesin (UniProt Q9BZZ2; also known as CD169, Sialic acid-binding Ig-like lectin 1, Sialic acid-binding immunoglobulin-like lectin 1, Siglec-1) is encoded by the SIGLEC1 (also known as SN) gene (Gene ID 6614) in human
- Sialoadhesin/CD169/Siglec-1 is the founding member of the sialic acid-binding immunoglobulin lectins (Siglec) expressed on a variety of leukocytes and stromal cells
- Sialoadhesin is initially produced with a signal sequence (a.a
- 1-19), the removal of which yields the mature protein with a large extracellular portion (a.a
- 20-1641), followed by a transmembrane segment (a.a
- 1642-1662), and a short cytoplasmic tail (a.a
- 1663-1709) with no known signal transduction or endocytosis motifs
- The extracellular portion contains an N-terminal Ig-like V-type domain (a.a
- 20-136), followed by sixteen Ig-like C2-type domains
- Sialoadhesin is highly expressed on the subcapsular sinus (SCS) and medullary macrophages in lymph node (LN) and on marginal metallophilic macrophages in the marginal zone (MZ) of spleen
- Although sialic acids are found on the surface of all cells and most secreted proteins, Only heavily sialylated, multimeric structures are captured by Sialoadhesin on macrophages via its N-terminal Ig-like V-type domain due to a low (millimolar) affinity toward sialylic acid
- Sialoadhesin-mediated sialic-acid recognition allows macrophages to engage sialylated pathogens and stimulate inflammatory responses
- Sialoadhesin is also reported to capture of B cell-derived exosomes via their surface-expressed α2, 3-linked sialic acids
- In the lymph node, exosomes are not retained in the subcapsular sinus of Siglec1-knockout mice and penetrate deeper into the paracortex, causing an enhanced response to antigen-pulsed exosomes among Siglec1-/- mice.