Tyrosinase Ab-1 Mouse Monoclonal Antibody, Epredia™

Description

• Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone
• Mutations of the tyrosinase gene occur in various forms of albinism
• Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients
• Host Species: Mouse Clone: T311 Isotype: IgG2a Species Reactivity: Human, Dog and Cat
• Weakly cross-reacts with Mouse
• Others not known
• Epitope: aa 1-433 Immunogen: Recombinant tyrosinase protein Molecular Weight: 70-80kDa Positive Control: Melanoma cell lines and Melanoma Cellular Localization: Cytoplasmic Ab-1 shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75
• Staining of melanomas with Ab-1 showed tyrosinase in melanotic as well as amelanotic variants
• Ab-1 is a useful marker for melanocytes and melanomas
• Occasionally a minor band at 55kDa is also detected in Western Blotting Recommended for: Immunohistochemistry (Formalin/paraffin) Western Blotting Immunofluorescence Immunocytology