Siglec-1/CD169 Antibody (ED3) - BSA Free, Novus Biologicals™

Siglec-1/CD169

Monoclonal

0.5 mg/mL

Flow Cytometry 1:500, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Frozen 1:50-1:250

CD169, FLJ00051, sialic acid binding Ig-like lectin 1, sialoadhesin, sialoadhesin

Mouse

Protein A purified

RUO

6614

Rat

Purified

Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Frozen)

ED3

Unconjugated

PBS (pH 7.4) with 0.09% Sodium Azide

SIGLEC1

Rat Spleen cell homogenate

0.025 mg

Primary

NB100-65301 recognizes the rat CD169 cell surface antigen, a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

IgG2a