MDM2/HDM2, p Ser185 Antibody, Novus Biologicals™

MDM2/HDM2 (p Ser185)

Polyclonal

Unconjugated

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide

ACTFS, HDM2, HDMX, MDM2 oncogene, E3 ubiquitin protein ligase

Rabbit

54.56 kDa

0.1 mg

Cancer, Cell Cycle and Replication, Hypoxia, Neuroscience, p53 Pathway, Phospho Specific, Tumor Suppressors

4193

Human, Mouse

IgG

Western Blot, ELISA, Immunoprecipitation

1 mg/ml

Western Blot 1:500-1:2000, ELISA 1:3000-1:12000, Immunoprecipitation 1:100

P23804

MDM2

This affinity purified MDM2/HDM2 [p Ser185] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 175-200 of mouse MDM2/HDM2/HDM2. (Uniprot: P23804)

Affinity Purified

RUO

Primary

This affinity-purified antibody is directed against the phosphorylated form of mouse MDM2/HDM2 protein at the pS185 residue. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against Mouse MDM2/HDM2 pS185 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated mouse MDM2/HDM2 is minimal by ELISA and western blot. A BLAST analysis was used to suggest minimal cross reactivity with MDM2/HDM2 homologues from other sources.

Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.