Notch-1 Antibody - (Cleaved N terminal), Novus Biologicals™

Notch-1

Polyclonal

Western Blot 1:500-1:2000, ELISA 1:20000-1:60000, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin 1:200

P46531

NOTCH1

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch-1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD). (Uniprot: P46531)

Unpurified

RUO

4851

Human, Mouse, Rat

Antisera

Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Unconjugated

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.1% Sodium Azide

EC 2.1.2.11, EC 3.4.21.68, hN1, Notch (Drosophila) homolog 1 (translocation-associated), notch 1Notch homolog 1, translocation-associated (Drosophila), Notch homolog 1, translocation-associated, TAN1neurogenic locus notch homolog protein 1, Translocation-associated notch protein TAN-1

Rabbit

272.5 kDa

0.2 mL

Primary

This antiserum is directed against human Notch-1. Based on the immunogen sequence, we expect this antibody to react as well with mouse and rat Notch-1 (100% sequence homology). This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.

Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.