NB600501

beta-Actin Antibody (AC-15), Novus Biologicals™

Manufacturer: Novus Biologicals

Select a Size

Pack Size SKU Availability Price
Each of 1 NB600501-Each-of-1 In Stock ₹ 58,562.00

NB600501 - Each of 1

₹ 58,562.00

In Stock

Quantity

1

Base Price: ₹ 58,562.00

GST (18%): ₹ 10,541.16

Total Price: ₹ 69,103.16

Antigen

beta-Actin

Classification

Monoclonal

Conjugate

Unconjugated

Gene Accession No.

P60709

Gene Symbols

ACTB

Immunogen

This beta-Actin Antibody (AC-15) was made to a slightly modified Beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Purification Method

Unpurified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Isotype

IgG1

Applications

ChIP Assay, ELISA, Flow Cytometry, Immunoassay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, KnockDown

Clone

AC-15

Dilution

Western Blot 1:5000 - 1:10000, Simple Western 1:25, Flow Cytometry, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:2000, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:500, Chromatin Immunoprecipitation (ChIP), Knockout Validated, KnockDown Validated

Gene Alias

ACTB, actin, beta, actin, cytoplasmic 1, anti-beta actin, B-Actin, Beta Actin, beta actin (ac-15), beta cytoskeletal actin, Beta-actin, beta-actin ac 15, beta-actin ac15, beta-actin ac-15, BRWS1, I(2)-Actin, PS1TP5-binding protein 1, PS1TP5BP1

Host Species

Mouse

Molecular Weight of Antigen

42 kDa

Quantity

0.1 mL

Research Discipline

Angiogenesis, Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Extracellular Matrix, Loading Controls, Signal Transduction, Stem Cell Markers

Gene ID (Entrez)

60

Target Species

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Feline, Fish, Guinea Pig, Hamster, Leech, Mammalia, Primate, Rabbit, Sheep, Squirrel, Turkey, Zebrafish, Drosophila (Negative)

Form

Ascites

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Description

  • beta-Actin Monoclonal specifically detects beta-Actin in Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Feline, Fish, Guinea Pig, Hamster, Leech, Mammal, Primate, Rabbit, Sheep, Squirrel, Turkey, Zebrafish, Drosophila (Negative) samples
  • It is validated for Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Chromatin Immunoprecipitation (ChIP), Knockout Validated, Knockdown Validated.