AKT1 Antibody, Novus Biologicals™

AKT1

Polyclonal

Western Blot 1:500-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1:2000-1:10000, Immunohistochemistry 1:500-1:2000, Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunohistochemistry-Paraffin 1:10-1:500, N/A

P31749, P31749, P31749, P31749

AKT1

AKT1 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human AKT1 proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. A BLAST analysis was used to suggest reactivity with this protein from rat, mouse, and chicken based on 100% homology for the immunogen sequence. (Uniprot: P31749)

Delipidation and Defibrination

RUO

Primary

Pan Anti-AKT1 Antibody reacts with the AKT1 from human tissues.

Store at -20°C. Avoid freeze-thaw cycles.

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Unconjugated

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide

AKT, AKT serine/threonine kinase 1, AKT1m, CWS6, EC 2.7.11, EC 2.7.11.1, PKB, PKB alpha, PKBMGC99656, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, rac protein kinase alpha, RAC-ALPHA, RAC-alpha serine/threonine-protein kinas

Rabbit

55.7 kDa

0.1 mL

Cancer, mTOR Pathway

207

Human, Mouse, Rat, Chicken

Antisera