Fibroblasts/Epithelial cells Antibody (D7-FIB), Alexa Fluor™ 532, Novus Biologicals™

Fibroblasts/Epithelial cells

Monoclonal

Alexa Fluor 532

50 mM sodium borate with 0.05% sodium azide

Human foreskin fibroblasts

Primary

Human

IgG2a

Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)

D7-FIB

Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen

Mouse

0.1 mL

NB600-777 recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes. D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (3). This product is routinely tested in flow cytometry on the KG1 cell line.

Store at 4°C in the dark.