L1/ORF2 Antibody, Novus Biologicals™

L1/ORF2

Polyclonal

Unconjugated

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide

EC=2.7.7.49, EC=3.1.21.-, Endonuclease, L1, LINE-1 retrotransposable element ORF2 protein, Long interspersed element-1, ORF2p, Retrovirus-related Pol polyprotein LINE-1, Reverse transcriptase

Chicken

149.01 kDa

0.1 mg

Primary

L1/ORF2 Antibody is directed against two regions within the endonuclease domain of L1 ORF2 protein. Reactivity occurs against human L1/ORF2 protein and is useful in determining its presence in various assays. A BLAST analysis was used to suggest cross reactivity with L1/ORF2 proteins from chimpanzee sources based on 100% homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88% homology with the immunizing sequences.

Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

Western Blot, ELISA

1 mg/ml

Western Blot 1:2000-1:10000, ELISA 1:10000

Q5BJF6

ODF2

This IgY fraction L1/ORF2 Antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein. (Uniprot: O00370)

Affinity Purified

RUO

4957

Human, Chimpanzee

IgG