IRS1, p Ser307 Antibody, Novus Biologicals™

IRS1 (p Ser307)

Polyclonal

Unconjugated

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide

HIRS-1, insulin receptor substrate 1, IRS-1

Rabbit

131.59 kDa

0.1 mg

Autophagy, Cancer, Diabetes Research, Lipid and Metabolism, mTOR Pathway, Nutrient Sensing in the Brain, Phospho Specific, Signal Transduction

3667

Human, Mouse, Rat, Monkey

IgG

Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)

1 mg/ml

Western Blot 1:250-1:1500, ELISA 1:2000-1:10000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500

P35568, P35568, P35568, P35568

IRS1

This affinity purified IRS1 [p Ser307] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 290-320 of human IRS1 protein. (Uniprot: P35568)

Affinity Purified

RUO

Primary

This affinity-purified antibody is directed against the phosphorylated form of human IRS1 protein at the pS307 residue. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human IRS1 pS307 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human IRS1 is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with IRS1 from human, mouse, rat, dog and vervet monkey based on 100% homology with the immunizing sequence. Partial reactivity is also expected against IRS1 from pig (94%), bovine (94%) and chicken (88%) sources. Reactivity of this antibody with IRS1 from other species is unknown.

Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.