Bcl-2 Antibody (100/D5), Novus Biologicals™

Bcl-2

Monoclonal

0.2mg/mL

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml

P10415

BCL2

The synthetic peptide corresponding to amino acids range from 41-64 (GAAPAPGIFSSQPG-Cys), of human Bcl-2 was used as immunogen to generate the antibody; GenBank no. NP_000648.2 (Pezzella et al, 1990).

Protein G purified

RUO

Primary

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Human, Mouse (Negative), Rat (Negative)

Purified

Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

100/D5

Unconjugated

PBS with 0.05% BSA. with 0.05% Sodium Azide

apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2

Mouse

25 kDa

0.2 mg

Apoptosis, Autophagy, Cancer, Cellular Markers, Mitochondrial Mediated Pathway, Mitophagy, Tumor Suppressors

596

Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.

Store at 4C.

IgG1 κ