Vimentin Antibody (VM452), Novus Biologicals™

Vimentin

Monoclonal

0.2 mg/ml

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml, Knockout Validated 0.7 ug/ml

FLJ36605, vimentin

Mouse

Protein A or G purified

RUO

Primary

This monoclonal antibody reacts with a 58kDa protein identified as Vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of Vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-Vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-Vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain Vimentin, e.g. hepatoma and seminoma. Anti-Vimentin is also useful as a tissue process control reagent.

Store at 4C.

IgG1 κ

Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

VM452

Unconjugated

P08670

VIM

Recombinant full-length human vimentin protein (Uniprot: P08670)

0.1 mg

Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Phospho Specific, Signal Transduction, Stem Cell Markers, Stem Cells

7431

Human, Porcine, Bovine, Canine, Chicken, Feline, Goat, Mouse (Negative), Rat (Negative)

Purified