p27/Kip1 Mouse, Clone: SX53G8, Novus Biologicals™

p27/Kip1

Monoclonal

Unconjugated

PBS with 0.05% BSA. with 0.05% Sodium Azide

CDKN4, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1), Cyclin-dependent kinase inhibitor p27, KIP1P27KIP1, MEN1B, MEN4, p27Kip1

Mouse

Protein A purified

RUO

Primary

This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.

Store at 4C.

IgG1 κ

Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

SX53G8

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml

P46527

CDKN1B

Purified GST-p27 fusion protein of human origin

0.2 mg

Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Phospho Specific, Prostate Cancer, Tumor Suppressors

1027

Human, Mouse, Rat, Primate

Purified