NBP232989B

PCNA Mouse, Clone: SPM350, Novus Biologicals™

Manufacturer: Fischer Scientific

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Antigen

PCNA

Classification

Monoclonal

Conjugate

Unconjugated

Formulation

PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias

cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Host Species

Mouse

Molecular Weight of Antigen

36 kDa

Quantity

0.2 mg

Research Discipline

Autophagy, Base Excision Repair, Cell Cycle and Replication, Cellular Markers, Core ESC Like Genes, DNA Polymerases, DNA Repair, Phospho Specific, Stem Cell Markers

Gene ID (Entrez)

5111

Target Species

Human, Mouse, Rat, Porcine, Chicken, Drosophila, Primate, Yeast, Zebrafish

Form

Purified

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Clone

SPM350

Dilution

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Gene Accession No.

P12004

Gene Symbols

PCNA

Immunogen

Rat PCNA/Protein A fusion protein

Purification Method

Protein A purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

Content And Storage

Store at 4C.

Isotype

IgG2a κ

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Description

  • PCNA Monoclonal specifically detects PCNA in Human, Mouse, Rat, Porcine, Chicken, Drosophila, Monkey, S
  • pombe, Yeast, Zebrafish samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.