Nucleolin Antibody (364-5 + NCL/902), Novus Biologicals™

Nucleolin

Monoclonal

0.2mg/mL

Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

P19338

NCL

Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902)

Protein A or G purified

RUO

Primary

Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Store at 4C.

IgG

Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

364-5 + NCL/902

Unconjugated

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Mouse

76 kDa

0.2 mg

Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers

4691

Human, Bovine (Negative), Mouse (Negative), Rat (Negative)

Purified