Cyclin B1 Antibody (SPM619), Novus Biologicals™

Cyclin B1

Monoclonal

0.2mg/mL

Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml

CCNB, cyclin B1, G2/mitotic-specific cyclin B1, G2/mitotic-specific cyclin-B1

Mouse

Protein A or G purified

RUO

Primary

It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Store at 4C.

IgG1 κ

Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

SPM619

Unconjugated

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

CCNB1

Recombinant human full-length CCNB1 protein

0.2 mg

Cancer, Cell Biology, Cell Cycle and Replication, Mitotic Regulators, Tumor Suppressors

891

Human, Mouse

Purified