TNF-alpha Antibody (J2D10) - Azide and BSA Free, Novus Biologicals™

TNF-alpha

Monoclonal

1.0 mg/mL

Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready

APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha

Recombinant human TNF-alpha

Protein A or G purified

RUO

Primary

This antibody neutralises HurTNFamediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

IgG1 κ

Flow Cytometry, Immunofluorescence, CyTOF

J2D10

Unconjugated

PBS with No Preservative

Mouse

17 kDa

0.1 mg

Adaptive Immunity, Asthma, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity

7124

Human, Rat, Mouse (Negative)

Purified