bcl-x Antibody (SPM337) - Azide and BSA Free, Novus Biologicals™
Manufacturer: Fischer Scientific
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Antigen
Bcl-xL
Classification
Monoclonal
Concentration
1.0 mg/mL
Dilution
Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias
Apoptosis regulator Bcl-X, bcl2-L-1, BCL2-like 1, bcl-2-like protein 1, BCLXBCL2LBcl-X, bcl-xL, BCLXL, BCL-XL/S, bcl-xS, BCLXS, DKFZp781P2092
Host Species
Mouse
Molecular Weight of Antigen
27 kDa
Quantity
0.2 mg
Research Discipline
Apoptosis, Cancer, Tumor Suppressors
Gene ID (Entrez)
598
Target Species
Human, Mouse, Rat, Porcine
Form
Purified
Applications
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone
SPM337
Conjugate
Unconjugated
Formulation
PBS with No Preservative
Gene Symbols
BCL2L1
Immunogen
A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein.
Purification Method
Protein A or G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype
IgG2a
Related Products
Description
- Bcl-xL Monoclonal specifically detects Bcl-xL in Human, Mouse, Rat, Porcine samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.