WT1 Antibody (WT1/857) - Azide and BSA Free, Novus Biologicals™

WT1

Monoclonal

1.0 mg/mL

Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

AWT1, NPHS4GUD, WAGR, Wilms tumor 1, Wilms tumor protein, WIT-2, WT33

Mouse

Protein A or G purified

RUO

Primary

Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors.WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

IgG1 κ

Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

WT1/857

Unconjugated

PBS with No Preservative

WT1

Recombinant human WT1 protein

0.1 mg

Angiogenesis, Cancer, Cell Cycle and Replication, Cellular Markers, Chromatin Research, Tumor Suppressors

7490

Human, Mouse, Rat

Purified