Cytochrome c Antibody (SPM389) - IHC-Prediluted, Novus Biologicals™

Cytochrome c

Monoclonal

Unconjugated

1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide

CYCS

Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c (Uniprot: P99999)

Protein A or G purified

RUO

Primary

It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This monoclonal antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

Store at 4C.

IgG2b κ

Immunohistochemistry (Paraffin)

SPM389

Immunohistochemistry-Paraffin

CYCHCS, cytochrome c, cytochrome c, somatic, THC4

Mouse

15 kDa

7 mL

Apoptosis, Cellular Markers, Cholesterol Metabolism, Core ESC Like Genes, Lipid and Metabolism, Mitochondrial Markers, Stem Cell Markers

54205

Human, Mouse, Rat, Amphibian, Avian, Canine, Drosophila, Equine

Purified