Diphtheria Toxin Polyclonal Antibody, HRP, Invitrogen™

Description

• The PA1-73138 antibody reacts with Diphtheria toxin
• PA1-73138 has been successfully used in ELISA applications
• The PA1-73138 antibody was raised against purified diphtheria toxoid
• Diphtheria toxin is a 58 kDa protein secreted by lysogenic strains of Corynebacterium diphtheriae
• The toxin causes the disease diphtheria in humans by gaining entry into the cell cytoplasm and inhibiting protein synthesis
• The mechanism of inhibition involves transfer of the ADP-ribose group of NAD to elongation factor-2 (EF-2), rendering EF-2 inactive
• The catalysed reaction is as follows: NAD + + peptide diphthamide = nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide The crystal structure of the diphtheria toxin homodimer has been determined to 2.5A resolution
• The structure reveals a Y-shaped molecule of 3 domains, a catalytic domain (fragment A), whose fold is of the alpha + beta type; a transmembrane (TM) domain, which consists of 9 alpha-helices, 2 pairs of which may participate in pH-triggered membrane insertion and translocation; and a receptor-binding domain, which forms a flattened beta-barrel with a jelly-roll-like topology
• The TM- and receptor binding-domains together constitute fragment B.