Alpha-Smooth Muscle Actin Recombinant Rabbit Monoclonal Antibody (17H19L35), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI701457-Each-of-1 | In Stock | ₹ 53,311.00 |
PI701457 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 53,311.00
GST (18%): ₹ 9,595.98
Total Price: ₹ 62,906.98
Antigen
Alpha-Smooth Muscle Actin
Classification
Recombinant Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide
Gene Accession No.
P62736, P62737, P62738
Gene Symbols
ACTA2
Immunogen
Peptide corresponding to amino acids 2-10 of human Smooth Muscle Actin
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Product Type
Antibody
Isotype
IgG
Applications
Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Clone
17H19L35
Conjugate
Unconjugated
Gene
ACTA2
Gene Alias
0610041G09Rik; AAT6; ACT-4; acta2; acta2.L; acta2.S; actin; Actin Alpha 2 Smooth Muscle Aorta; actin alpha 2, smooth muscle; Actin Vascular Smooth Muscle; actin, alpha 2, smooth muscle, aorta; actin, alpha 2, smooth muscle, aorta L homeolog; actin, alpha 2, smooth muscle, aorta S homeolog; actin, alpha, vascular smooth muscle; actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; ACTSA; ACTVS; alpha actin 2; Alpha Cardiac Actin; alpha smooth muscle actin; alpha-actin; Alpha-actin-2; alpha-cardiac actin; alphaSMA; alpha-SM-actin; alpha-smooth muscle actin; Aortic Smooth Muscle; ASMA; a-sma; cell growth-inhibiting gene 46 protein; GIG46; Growth Inhibiting Gene 46; hypothetical protein LOC515610; LOW QUALITY PROTEIN: actin, aortic smooth muscle; mymy5; SM actin; sma; SMactin; sm-actin; SMalphaA; smooth muscle; smooth muscle alpha-actin; vascular smooth muscle alpha-actin; wu:fb63d03; XELAEV_18034370mg
Host Species
Rabbit
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
11475, 59, 81633
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
Related Products
Description
- This antibody is predicted to react with bovine, canine, rabbit, rat and porcine based on sequence homology
- Intact IgG appears on a non-reducing gel as ∽150 kDa band and upon reduction generating a ∽25 kDa light chain band and a ∽50 kDa heavy chain
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
- The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
- Then, individual clones are screened to select the best candidates for production
- The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
- Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity
- Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility
- In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle
- Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton
- Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis
- Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands
- Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6
- Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates
- Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells
- In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases
- Multiple alternatively spliced variants of smooth muscle actin have been identified.