PIMA1202
Cas9 Monoclonal Antibody (10C11-A12), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIMA1202-Each-of-1 | In Stock | ₹ 50,018.00 |
PIMA1202 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 50,018.00
GST (18%): ₹ 9,003.24
Total Price: ₹ 59,021.24
Antigen
Cas9
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide
Gene Accession No.
Q99ZW2
Gene Symbols
cas9
Immunogen
Cas9 nuclease
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Bacteria
Product Type
Antibody
Isotype
IgG1 κ
Applications
Flow Cytometry, Immunocytochemistry, Western Blot
Clone
10C11-A12
Conjugate
Unconjugated
Gene
cas9
Gene Alias
cas9; CASP 9; Caspase9; Caspase-9 precursor; CASPASE-9c; CRISPR9; CRISPR-Cas9; CTD; OTTHUMP00000002324; RNCASP9; RP11-265F14.3; s aureus
Host Species
Mouse
Purification Method
Protein G
Regulatory Status
RUO
Gene ID (Entrez)
901176
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Form
Liquid
Related Products
Description
- Cas9 (10C11-A12) Mouse mAb recognizes transfected levels of total Cas9 protein
- Stable Cas9 U2OS cell line used in validation experiments expresses Cas9 at lower levels
- Assay results may vary depending on expression level of Cas9 in cell lines tested
- This product was tested in Streptococcus pyogenes bacteria
- Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering
- The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence
- It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome
- In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation
- Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9)
- SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity
- SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides
- In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM
- This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.