PIMA181890
BrdU Monoclonal Antibody (BU20A), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIMA181890-Each-of-1 | In Stock | ₹ 50,774.50 |
PIMA181890 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 50,774.50
GST (18%): ₹ 9,139.41
Total Price: ₹ 59,913.91
Antigen
BrdU
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.4
Host Species
Mouse
Purification Method
Protein A
Regulatory Status
RUO
Target Species
Chemical
Product Type
Antibody
Isotype
IgG1
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
Clone
BU20A
Conjugate
Unconjugated
Gene Alias
5-Bromo-2-deoxyuridine; Bromodeoxyuridine
Immunogen
Bromodeoxyuridine conjugated to BSA.
Quantity
200 μg
Primary or Secondary
Primary
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
Target Molecule
Chemical
Related Products
Description
- For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL
- Mouse anti BrdU antibody, clone Bu20a recognizes bromodeoxyuridine (known as BrdU or BrdUrd)
- Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine
- BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication
- Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA
- Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.