BrdU Monoclonal Antibody (BU-1), Invitrogen™

Description

• MA3-071 detects 5-Bromo-2-deoxyuridine (BrdU) in many samples
• MA3-071 has been successfully used in immunofluorescence, immunohistochemistry, and flow cytometry applications
• The MA3-071 immunogen is 5-iodouridine covalently coupled to ovalbumin
• This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit
• The antibody sold separately may have slightly different performance and may need to be further optimized for the best results
• Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine
• BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication
• Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA
• Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.