ICAD Monoclonal Antibody (OTI1B10), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIMA524888-Each-of-1 | In Stock | ₹ 52,198.50 |
PIMA524888 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 52,198.50
GST (18%): ₹ 9,395.73
Total Price: ₹ 61,594.23
Antigen
ICAD
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
PBS with 1% BSA, 50% glycerol and 0.02% sodium azide; pH 7.3
Gene Accession No.
O00273
Gene Symbols
DFFA
Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 1-331 of DFF45.
Quantity
100μL
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1
Applications
Immunocytochemistry, Western Blot
Clone
OTI1B10
Conjugate
Unconjugated
Gene
DFFA
Gene Alias
A330085O09Rik; DFF; DFF1; DFF35; Dff45; DFF-45; Dffa; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45kDa, alpha polypeptide; DNA fragmentation factor, alpha subunit; H13; ICAD; ICAD-L; ICAD-S; Inhibitor of CAD; RP23-121D17.3
Host Species
Mouse
Purification Method
Affinity Chromatography
Regulatory Status
RUO
Gene ID (Entrez)
1676
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Form
Liquid
Related Products
Description
- ICAD is the inhibitor of caspase-3-activated DNase which is a substrate that controls nuclear apoptosis
- ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45; and a 35kDa isoform, ICAD-S/DFF35
- Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional
- ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation
- In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD
- The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD
- In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone
- There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells
- Two alternatively spliced transcript variants encoding distinct isoforms have been found for the ICAD gene.