TPH1 Recombinant Rabbit Monoclonal Antibody (145), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIMA529618-Each-of-1 | In Stock | ₹ 41,385.00 |
PIMA529618 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 41,385.00
GST (18%): ₹ 7,449.30
Total Price: ₹ 48,834.30
Antigen
TPH1
Classification
Recombinant Monoclonal
Concentration
5.0 mg/mL
Formulation
PBS with no preservative
Gene Accession No.
P17752
Gene Symbols
TPH1
Immunogen
Recombinant Human Tryptophan Hydroxylase 1/TPH1 Protein (Ile2-Ile444)
Quantity
100μL
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG
Applications
Immunoprecipitation, Western Blot
Clone
145
Conjugate
Unconjugated
Gene
TPH1
Gene Alias
indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; MGC119994; TPH; Tph1; TPH2; TPRH; TRPH; tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; tryptophan hydroxylase; tryptophan hydroxylase (tryptophan 5-monooxygenase); tryptophan hydroxylase 1; tryptophan hydroxylase 1 (tryptophan 5-monooxygenase)
Host Species
Rabbit
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
7166
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
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Description
- This product is preservative free
- It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%)
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
- The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
- Then, individual clones are screened to select the best candidates for production
- The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
- This antibody has specificity for Human Tryptophan Hydroxylase 1/TPH1
- Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001)
- In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland
- Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis
- Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland
- The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II) (Jiang et al., 2000; Johansen et al., 1996)
- Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).