PIMA533091
CD163 Recombinant Rabbit Monoclonal Antibody (RM371), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIMA533091-Each-of-1 | In Stock | ₹ 47,481.50 |
PIMA533091 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 47,481.50
GST (18%): ₹ 8,546.67
Total Price: ₹ 56,028.17
Antigen
CD163
Classification
Recombinant Monoclonal
Concentration
0.2 mg/mL
Formulation
PBS with 1% BSA, 50% glycerol and 0.09% sodium azide
Gene Accession No.
Q86VB7
Gene Symbols
CD163
Immunogen
A peptide corresponding to the N-terminus of human CD163.
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG
Applications
Immunohistochemistry (Paraffin), Western Blot
Clone
RM371
Conjugate
Unconjugated
Gene
CD163
Gene Alias
CD163, CD163 antigen, CD163 molecule, CD163v2, CD163v3, Hemoglobin scavenger receptor, M130, macrophage-associated antigen, MM130, putative CD163 antigen, SCARI1, Scavenger receptor cysteine-rich type 1 protein M130, sCD163, Soluble CD163, Soluble sCD163
Host Species
Rabbit
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
9332
Content And Storage
-20°C
Form
Liquid
Related Products
Description
- Specificity: reacts to human CD163
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems
- The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits
- Then, individual clones are screened to select the best candidates for production
- The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
- CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex
- CD163 protects tissues from free hemoglobin-mediated oxidative damage, and may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme
- CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation
- Specifically, CD163 is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan's cells
- CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes
- CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone
- Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant
- CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, and exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype
- Further, CD163 also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.