Ebola Virus VP40 (subtype Sudan, Zaire) Monoclonal Antibody (2614), Invitrogen™

Description

• Specificity: Reactive with the VP40 of Sudan & Zaire sequences
• Reactive with VLPs
• Purified preparations consist of >90% pure mouse monoclonal antibody which has been purified from ascites fluid by protein A chromatography
• The final preparation is formulated to a protein concentration of 100 μg/mL in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide
• Each vial contains 1.0 mL
• This product contains no stabilizing proteins and should be stored at 4C until ready for use
• Working dilution must be determined by the user
• Suggested starting ranges are 1:10-1:50 for ICC/IF and 1:20-1:200
• for ELISA
• Ebola virus is an enveloped, negative-stranded RNA virus
• This virus belongs to the family Filoviridae and has a distinctive morphology similar only to that of Marburg virus, but apparently has no antigenic characteristics of Marburg virus
• Its genome is a nonsegmented RNA strand
• SDS-PAGE profiles demonstrate the presence of seven structural proteins
• These are the major nucleoprotein NP (104 K), glycoprotein GP (125K), L protein (180 K), and four proteins: VP40 (40 K), ViPX (35 K), VP30 (30 K) and VP24 (24 K)
• All the proteins are encoded by monocistronic mRNA transcripts complementary to virion RNA
• GP is the major protein of the surface spikes and only this 125K viral protein is glycosylated.