Phospho-ATF6 (Thr166) Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA5105245-Each-of-1 | In Stock | ₹ 45,345.50 |
PIPA5105245 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 45,345.50
GST (18%): ₹ 8,162.19
Total Price: ₹ 53,507.69
Antigen
Phospho-ATF6 (Thr166)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
ATF6
Gene Alias
9130025P16Rik; 9630036G24; AA617266; AA789574; ACHM7; activating transcription factor 6; activating transcription factor 6 alpha; Activating transcription factor 6 beta; ATF 6; Atf6; ATF6 alpha; ATF6A; Atf6alpha; ATF6-alpha; Atf6b; ATF6beta; ATF6-beta; cAMP response element binding protein-related protein; cAMP response element-binding protein-related protein; cAMP responsive element binding protein-like 1; cAMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 beta; cAMP-responsive element-binding protein-like 1; Crebl1; Creb-related protein; CREB-RP; Cyclic AMP-dependent transcription factor ATF-6 alpha; cyclic AMP-dependent transcription factor ATF-6 beta; DKFZp686P2194; ESTM49; FLJ21663; G13; Processed cyclic AMP-dependent transcription factor ATF-6 alpha; Processed cyclic AMP-dependent transcription factor ATF-6 beta; Protein G13
Host Species
Rabbit
Purification Method
sequential chromatography
Regulatory Status
RUO
Gene ID (Entrez)
12915, 1388, 226641, 22926, 304962, 406169
Content And Storage
-20°C
Form
Liquid
Applications
Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Concentration
1 mg/mL
Formulation
PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
Gene Accession No.
F6VAN0, G3V909, O35451, P18850, Q99941
Gene Symbols
ATF6, ATF6B
Immunogen
A synthesized peptide derived from human ATF6(Accession P18850), corresponding to amino acid residues around phosphorylated Thr166.
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Product Type
Antibody
Isotype
IgG
Related Products
Description
- Antibody detects endogenous levels of ATF6 only when phosphorylated at Thr166
- ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes
- It binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3')
- Binding to ERSE requires binding of NF-Y to ERSE
- ATF6 could also be involved in activation of transcription by the serum response factor
- ATF6 exists as a homodimer and heterodimer with ATF6 beta
- The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC)
- It Interacts also with the transcription factors GTF2I, YY1 and SRF
- Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus
- The basic domain of ATF6 functions as a nuclear localization signal and the basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE
- During the unfolded protein response an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis
- The cleavage seems to be performed sequentially by site-1 and site-2 proteases
- ATF6 is N-glycosylated, phosphorylated in vitro by MAPK14/P38MAPK and belongs to the bZIP family.