Histone H2A.X Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA5121418-Each-of-1 | In Stock | ₹ 44,811.50 |
PIPA5121418 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 44,811.50
GST (18%): ₹ 8,066.07
Total Price: ₹ 52,877.57
Antigen
Histone H2A.X
Classification
Polyclonal
Conjugate
Unconjugated
Gene
H2AX
Gene Alias
AW228881; gamma H2AX; gammaH2ax; H2A histone family member X; H2A histone family, member X; H2A.X; H2A.X variant histone; H2A/X; h2afx; H2ax; H2AX histone; Hist5-2ax; histone 5 protein 2ax; histone H2A.x; Histone H2a/x; Histone H2AX; RGD1566119; similar to H2A histone family, member X; zgc:56329
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
3014
Content And Storage
-20°C or -80°C if preferred
Form
Liquid
Applications
ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
Concentration
0.04 mg/mL
Formulation
PBS with 50% glycerol and 0.03% ProClin 300; pH 7.4
Gene Accession No.
P16104
Gene Symbols
H2AX
Immunogen
Synthesized peptide derived from Human Histone H2AX protein (130-142aa)
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG
Related Products
Description
- Antibody tested in Indirect ELISA Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle
- In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX
- The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs
- The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner
- Structurally, H2A.x contains 143 amino acid residues
- Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs
- There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA
- The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA
- Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome
- Histone H2A.x contributes to histone-formation and therefore the structure of DNA
- Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.