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PIPA519891

DFFB Polyclonal Antibody, Invitrogen™

Manufacturer: Thermo Scientific

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Pack Size SKU Availability Price
Each of 1 PIPA519891-Each-of-1 In Stock ₹ 45,968.50

PIPA519891 - Each of 1

₹ 45,968.50

In Stock

Quantity

1

Base Price: ₹ 45,968.50

GST (18%): ₹ 8,274.33

Total Price: ₹ 54,242.83

Antigen

DFFB

Classification

Polyclonal

Conjugate

Unconjugated

Gene

DFFB

Gene Alias

2410008J01Rik; 40kDa; 5730477D02Rik; AU018859; CAD; caspase-activated deoxyribonuclease; caspase-activated DNase; caspase-activated nuclease; Cpad; CPAN; DFF2; DFF-2; DFF40; DFF-40; DFFB; Didff; DNA fragmentation factor 40 kD beta polypeptide (caspase-activated DNase); DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40 kD, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, 40 kD, beta subunit; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, beta polypeptide (caspase-activated DNase); DNA fragmentation factor, beta subunit; DNase inhibited by DNA fragmentation factor; RP13-531C17.1; RP23-254N4.2

Host Species

Rabbit

Purification Method

Antigen affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

13368, 84359

Content And Storage

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Form

Liquid

Applications

Immunohistochemistry, Western Blot

Concentration

1.0 mg/mL

Formulation

PBS with 0.02% sodium azide

Gene Accession No.

O54788, Q99N34

Gene Symbols

DFFB

Immunogen

A peptide corresponding to 17 amino acids near the center of murine CAD.

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Mouse, Rat

Product Type

Antibody

Isotype

IgG

Description

  • In Western blot applications, this antibody detects a band at ∽40kDa
  • A suggested positive control is mouse kidney tissue lysate
  • PA5-19891 can be used with blocking peptide PEP-0019
  • Caspase-activated DNase (CAD) cleaves chromosomal DNA during apoptosis
  • Apoptosis which is a cell death process removing toxic and/or useless cells during mammalian development can be induced by a variety of stimuli, including the death factors of the tumor necrosis factor family, or UV irradiation, anticancer drugs, and survival factor deprivation
  • The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units
  • CAD, which can be activated by caspases, exists as a complex with its inhibitor, inhibitor of CAD (ICAD)
  • ICAD helps correct folding of CAD and remains complexed with CAD to inhibit the DNase activity of CAD
  • Caspase, which is activated during the apoptotic process, cleaves ICAD, and the CAD released from ICAD enters the nuclei to cleave the chromosomal DNA
  • Internal as well as external signals can activate caspases and this is under the control of the balance between pro-and anti-apoptotic proteins of the Bcl-2 family, heat shock proteins, and inhibitors of apoptosis proteins (IAPs)
  • Currently, fourteen members of the caspase family have been identified, of which seven mediate apoptosis.