BCKDHA Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA529300-Each-of-1 | In Stock | ₹ 52,243.00 |
PIPA529300 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 52,243.00
GST (18%): ₹ 9,403.74
Total Price: ₹ 61,646.74
Antigen
BCKDHA
Classification
Polyclonal
Conjugate
Unconjugated
Gene
BCKDHA
Gene Alias
2-oxoisovalerate dehydrogenase (lipoamide); 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; BCKAD E1[a]; BCKDA; BCKDE1A; BCKDH E1-alpha; BCKDHA; branched chain alpha-ketoacid dehydrogenase subunit E1 alpha; branched chain keto acid dehydrogenase E1 alpha protein; branched chain keto acid dehydrogenase E1, alpha polypeptide; branched chain keto acid dehydrogenase subunit E1, alpha polypeptide; branched chain ketoacid dehydrogenase E1, alpha polypeptide; branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; E1a; MSU; MSUD1; OVD1A
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
593
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
Applications
Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Concentration
1 mg/mL
Formulation
PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
Gene Accession No.
P12694
Gene Symbols
BCKDHA
Immunogen
Recombinant fragment corresponding to a region within amino acids 252 and 445 of Human BCKDHA
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG
Related Products
Description
- Recommended positive controls: A549, HeLa, HepG2
- Predicted reactivity: Mouse (95%), Rat (95%), Pig (94%), Sheep (95%), Chimpanzee (98%), Bovine (94%)
- Store product as a concentrated solution
- Centrifuge briefly prior to opening the vial
- The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of the branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine
- This reaction is the second major step in the catabolism of the branched-chain amino acids (Wynn et al., 1998)
- The BCKD complex consists of 3 catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase (E2), and a homodimeric dihydrolipoamide dehydrogenase (E3)
- E1 is a thiamine pyrophosphate (TPP)-dependent enzyme
- The reaction is irreversible and constitutes the first committed step in BCAA oxidation
- The BCKDHB gene encodes the beta subunit of E1
- The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase.