PIPA532256
BrdU Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA532256-Each-of-1 | In Stock | ₹ 49,439.50 |
PIPA532256 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 49,439.50
GST (18%): ₹ 8,899.11
Total Price: ₹ 58,338.61
Antigen
BrdU
Classification
Polyclonal
Conjugate
Unconjugated
Gene Alias
5-Bromo-2-deoxyuridine; Bromodeoxyuridine
Immunogen
BrdU conjugated to KLH
Quantity
100 μL
Primary or Secondary
Primary
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
Target Molecule
Chemical
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
Concentration
1 mg/mL
Formulation
PBS with 20% glycerol and 0.025% ProClin 300; pH 7
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Target Species
Chemical
Product Type
Antibody
Isotype
IgG
Related Products
Description
- Recommended positive controls: HeLa cells were treated with 100uM BrdU for 4hrs, U343 cells were treated with 10 μM BrdU for 18hrs
- Store product as a concentrated solution
- Centrifuge briefly prior to opening the vial
- Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine
- BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication
- Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA
- Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.