F4/80 Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA532399-Each-of-1 | In Stock | ₹ 51,575.50 |
PIPA532399 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 51,575.50
GST (18%): ₹ 9,283.59
Total Price: ₹ 60,859.09
Antigen
F4/80
Classification
Polyclonal
Conjugate
Unconjugated
Gene
ADGRE1
Gene Alias
ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
13733
Content And Storage
4° C, do not freeze
Form
Liquid
Applications
Immunohistochemistry (Paraffin)
Concentration
Conc. Not Determined
Formulation
PBS with 1% BSA and 0.1% sodium azide; pH 7.2
Gene Accession No.
Q61549
Gene Symbols
ADGRE1
Immunogen
Synthetic peptide derived from N-terminus of mouse F4/80 protein.
Quantity
500 μL
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG
Related Products
Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min
- Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature
- A suggested positive control is human lung or mouse liver tissue
- F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1)
- Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation
- The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen
- F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node
- The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.