Calnexin Polyclonal Antibody, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIPA534754-Each-of-1 | In Stock | ₹ 57,138.00 |
PIPA534754 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 57,138.00
GST (18%): ₹ 10,284.84
Total Price: ₹ 67,422.84
Antigen
Calnexin
Classification
Polyclonal
Conjugate
Unconjugated
Gene
CANX
Gene Alias
1110069N15Rik; AI988026; calmegin; calnexin; canx; canx-a; canx-b; clgn; clnx; cnx; D11Ertd153e; FLJ26570; hypothetical protein; IP90; Major histocompatibility complex class I antigen-binding protein p88; p88; P90; PP90; pp90 precursor
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
101122273, 12330, 29144, 821
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
Applications
Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Concentration
0.46 mg/mL
Formulation
PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
Gene Accession No.
P27824, P35564, P35565
Gene Symbols
CANX
Immunogen
Synthetic peptide corresponding to a region within amino acids 525 and 592 of Calnexin (Uniprot ID#P27824)
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse, Ovine, Rat
Product Type
Antibody
Isotype
IgG
Related Products
Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, A375, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2, KN73
- Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Pig (100%), Bovine (100%)
- IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Store product as a concentrated solution
- Centrifuge briefly prior to opening the vial
- Calnexin, also referred to as IP90, p88 and p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum (ER)
- Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins
- Studies indicate that calnexin associates with the major histocompatibility complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes
- It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER
- The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase
- Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention
- Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids).