R94025
HisG Epitope Tag Monoclonal Antibody, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | R94025-Each-of-1 | In Stock | ₹ 29,281.00 |
R94025 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 29,281.00
GST (18%): ₹ 5,270.58
Total Price: ₹ 34,551.58
Antigen
HisG Epitope Tag
Classification
Monoclonal
Formulation
PBS with 0.01% sodium azide
Host Species
Mouse
Purification Method
purified
Regulatory Status
RUO
Target Species
Tag
Product Type
Antibody
Isotype
IgG2a
Applications
ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Gene Alias
6xHis-Gly; 6xHis-Glycine; His G; His Tag; Histidine Tag
Immunogen
6x His synthetic peptide: -His-His-His-His-His-His-Gly
Quantity
50 μL
Primary or Secondary
Primary
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
Target Molecule
Tag
Related Products
Description
- NOTE: R940-25 was previously sold under product #46-1008
- When re-ordering, please use #R940-25
- This antibody is designed to specifically detect recombinant proteins containing the seven amino acid 6xHis-Gly epitope
- This epitope is present in many expression vectors encoding an N-terminal 6xHis tag
- The HisG antibody recognizes the following sequence: -His-His-His-His-His-His-Gly
- This product contains enough material for 25 Western blots
- It has been tested against purified Positope™ control protein (100 ng)
- The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-myc, and V5
- For Western blot, dilute in PBS containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM)
- Using chemiluminescence as the detection method, no cross-reactivity has been observed in bacterial lysates
- In mammalian lysates, a few cross-reactive proteins have been observed upon overexposure of blots
- This antibody has also been used successfully to immunoprecipitate fusion proteins that contain the 6xHis tag
- Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available
- Expression of recombinant proteins in E
- coli as a fusion protein with neighboring histidine residues is one of the most popular methods of epitope tags
- The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent
- HisG antibodies allow detection of recombinant proteins containing a polyhistidine sequence: His-His-His-His-His-His-Gly (6xHis-Gly epitope)
- HisG antibodies can be used to detect expression of fusion proteins from bacterial, insect, and mammalian cells.