40718
Diethyl pyrocarbonate
96% (NT)
Manufacturer: Sigma Aldrich
CAS Number: 1609-47-8
Synonym(S): DEP, DEPC, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 5 ML | 40718-5-ML | In Stock | ₹ 9,590.95 |
| 25 ML | 40718-25-ML | In Stock | ₹ 21,628.35 |
| 100 ML | 40718-100-ML | In Stock | ₹ 70,135.18 |
| 250 ML | 40718-250-ML | In Stock | ₹ 1,00,802.40 |
40718 - 5 ML
In Stock
Quantity
1
Base Price: ₹ 9,590.95
GST (18%): ₹ 1,726.371
Total Price: ₹ 11,317.321
Quality Level
200
Assay
96% (NT)
form
liquid
color
APHA: ≤20
refractive index
n20/D 1.398 (lit.)n20/D 1.398
bp
93-94 °C/18 mmHg (lit.)
solubility
95% ethanol: 4.5 g/10 mL, clear, colorless
density
1.121 g/mL at 20 °C1.101 g/mL at 25 °C (lit.)
shipped in
wet ice
storage temp.
2-8°C
Other Options
Related Products
Description
- General description: Diethyl pyrocarbonate (DEP) is a bactericidal agent and decomposes in water to CO2 and ethanol. DEP inhibits enzymes such as DNase and RNase without affecting nucleic acids. It is useful for the preparation of mRNA from tissues.[1]
- Application: Diethyl pyrocarbonate may be used in the following studies:For the modification of histidyl residues in proteins.[2]As a nuclease inhibitor, for the extraction of undegraded nucleic acids from etiolated and green plant tissues.[3]For the modification of linear and supercoiled plasmid DNAs.[4]As a chemical probe to investigate the secondary structure in negatively supercoiled DNA.[4]For cabethoxylation of histidine residues of actin.[5]
- Biochem/physiol Actions: Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.
SAFETY INFORMATION
Pictograms
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral
WGK
WGK 3
Flash Point(F)
closed cup
Flash Point(C)
closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves