860336

3,3′,5,5′-Tetramethylbenzidine

≥99%

Manufacturer: Sigma Aldrich

CAS Number: 54827-17-7

Synonym(S): BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate

Select a Size

Pack Size SKU Availability Price
100 MG 860336-100-MG In Stock ₹ 2,316.55
1 G 860336-1-G In Stock ₹ 6,830.58
5 G 860336-5-G In Stock ₹ 22,840.75
25 G 860336-25-G In Stock ₹ 46,038.73

860336 - 100 MG

₹ 2,316.55

In Stock

Quantity

1

Base Price: ₹ 2,316.55

GST (18%): ₹ 416.979

Total Price: ₹ 2,733.529

Quality Level

200

Assay

≥99%

form

powder

mp

168-171 °C (lit.)

storage temp.

2-8°C

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

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Description

  • General description: 3,3′,5,5′-Tetramethylbenzidine is a peroxidase sensitive dye.[1] It is a better alternative to syringaldazine for the determination of chlorine in water due to its product stability and no interference from calcium and magnesium ions.[2]
  • Application: 3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

SAFETY INFORMATION

Pictograms

GHS08,GHS07

Signal Word

Warning

Hazard Statements

H302,H351,H413

Precautionary Statements

P202 - P264 - P273 - P280 - P301 + P312 - P308 + P313

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves