H1759

(+)-Biotin N-hydroxysuccinimide ester

≥98% (HPLC), powder

Manufacturer: Sigma Aldrich

CAS Number: 35013-72-0

Synonym(S): (+)-Biotin N-succinimidyl ester, d-Biotin NHS ester, BNHS, Biotinyl-N-hydroxy-succinimide, N-Hydroxysuccinimidobiotin, N-Succinimidyl D-biotinate, NHS-Biotin

Select a Size

Pack Size SKU Availability Price
5 MG H1759-5-MG In Stock ₹ 2,998.53
25 MG H1759-25-MG In Stock ₹ 7,317.70
100 MG H1759-100-MG In Stock ₹ 20,394.30
250 MG H1759-250-MG In Stock ₹ 38,407.10
1 G H1759-1-G In Stock ₹ 97,425.00

H1759 - 5 MG

₹ 2,998.53

In Stock

Quantity

1

Base Price: ₹ 2,998.53

GST (18%): ₹ 539.735

Total Price: ₹ 3,538.265

Quality Level

200

Assay

≥98% (HPLC)

form

powder

reaction suitability

reaction type: Biotinylations

color

white to off-white

mp

212 °C

solubility

DMF: ≤50 mg/mL (Stable for at least one month in dry DMF)DMSO: soluble

storage temp.

−20°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(=O)ON3C(=O)CCC3=O)[C@@]1([H])NC(=O)N2

InChI

1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1

Description

  • Application: (+) Biotin NHS ester or (+)-Biotin N-hydroxysuccinimide ester has been used: to biotinylate Hyb 246-4 for surfactant protein D enzyme-linked immunosorbent assay (SP-D ELISA)[1]to biotinylate proteins to form preformed complexes[2]to label and modify monoclonal anti-microfibrillar-associated protein 4 (MFAP4) (HG-HYB 7-18) to bind to alphaLISA streptavidin-coated donor beads[3]
  • Biochem/physiol Actions: The N-hydroxysuccinimide ester (NHS)-biotin modification helps in the biotinylation of the target lysine residue at its ε-amino group and identifies the surface lysine residues. It is used as a tool to identify the interaction of proteins with several biological molecules, like protein-polysaccharide interactions, protein-protein interactions, protein-nucleic acid interactions, and protein-ligand interactions.[4] NHS-biotin may be used in the biotinylation of proteins and peptides. It is typically coupled to primary amine in the pH range of 6.5-8.5.
  • Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

SAFETY INFORMATION

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves