Description

• General description: Phosphoramidite technology is ideally suited for the modification ofsynthetic oligonucleotides with covalently attached reporter moieties,linkers, spacers, or haptens. Sigma-Aldrich provides popular reporters andlinkers ready to use for DNA/RNA synthesis machines such as β-cyanoethylphosphoramidites. 6-Fluorescein Phosphoramidite is one such label whose absorbance maxima is 496 nm and emission maxima is 520 nm.Key Features of 6-Fluorescein Phosphoramidite:6-Fluorescein Phosphoramidite consists of one isomer derived from6-carboxy fluorescein Couples to the 5′-end of the oligonucleotide using standard synthesisprotocols Protecting groups on the fluorescein moiety are removed under standardconditions of cleavage and deprotection with concentrated ammoniaA detritylation step is not required since the fluorescein phosphoramiditedoes not contain a DMT-groupThe labeled oligonucleotide is ready for use in most applications after theevaporation of ammonia. Standard procedures can be used if additionalpurification is required.Lot-to-lot consistent purity and performanceManufactured under a certified ISO 9001 quality system6-Fluorescein Phosphoramidite is configured for ABI Synthesizers.