K278001
Invitrogen™ Calcium Phosphate Transfection Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K278001-Each-of-1 | In Stock | ₹ 60,164.00 |
K278001 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 60,164.00
GST (18%): ₹ 10,829.52
Total Price: ₹ 70,993.52
Content And Storage
2 × 12 ml Tissue Culture Sterile Water2 × 12 ml 2X HEPES Buffered Saline (HBS)3 × 1 ml 2 M CaCl220 μg pcDNA™3.1/His/lacZStore all components at -5 to -30°C.
Format
6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Transfection Technique
Chemical Transfection
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
75 Reactions
Cell Type
Established Cell Lines
Sample Type
Plasmid DNA
For Use With (Application)
Transfection
Product Type
Transfection Kit
Serum Compatible
No
Related Products
Description
- Based on forming a calcium phosphate-DNA precipitate Facilitates the binding of the DNA to the cell surface
- DNA then enters the cell by endocytosis First developed by Graham and van der Ebb and was later modified by Wigle Used to transfect a wide variety of cell types for transient expression or for producing stable transformants DNA is mixed directly with a concentrated solution of CaCl 2 , which is then added dropwise to a phosphate buffer to form a fine precipitate Aeration of the phosphate buffer while adding the DNA-CaCl 2 solution helps to ensure that the precipitate that forms is as fine as possible, which is important because clumped DNA will not adhere to or enter the cell as efficiently Product size: 75 reactions Cell Culture, Plasmid Transfection, Transfection Order Info Shipping Condition: Room Temperature