Molecular Probes™ pHrodo™ GreenS. aureusBioParticles™ Phagocytosis Kit for Flow Cytometry

Description

• The pHrodo™ Green S
• aureus BioParticles™ Phagocytosis Kit for Flow Cytometry enables the detection of phagocytic activity in whole blood samples and live cell lines by flow cytometry
• The kit includes all the reagents required for assessing particle ingestion and red blood cell lysis
• Sufficient reagents are provided in the kit for performing approximately 100 assays using 100 μL of whole blood per assay
• View a selection guide for all pHrodo Indicators used in imaging, flow cytometry and microplate assays
• The pHrodo™ Green S
• aureus BioParticles™ assay provides sensitive detection without the need for quenching reagents and extra wash steps, helping save time and minimize the uncertainty of whether particle signals derive from internalized particles
• The pHrodo™ Green S
• aureus BioParticles™ Phagocytosis Kit is: • Specific—fluorogenic, pH-sensitive dye for detection of phagocytosis • Rapid and reproducible—no wash steps or quencher dye required • Flexible—multiplex with reagents excited by other laser lines • Compatible—multiplex with standard reagents and CD markers How It works The pHrodo™ Green S
• aureus BioParticles™ conjugates are inactivated, unopsonized S
• aureus which function as highly sensitive, fluorogenic particles for the detection of phagocytic ingestion
• The unique pHrodo™ dye-based system measures phagocytic activity based on acidification of particles as they are ingested, eliminating the wash and quenching steps that are necessary with nonfluorogenic indicators of bacterial uptake
• The pHrodo™ Green Dye The new Molecular Probes™ proprietary, pH-sensitive, pHrodo™ Green dye is non-fluorescent at neutral pH, but turns bright green upon acidification
• Because it is both fluorogenic and pH-sensitive, the pHrodo™ Green dye can be used as a specific sensor of phagocytic events; acidification of the phagosome following phagocytosis is marked by green fluorescence
• It is therefore an ideal tool with which to study phagocytosis and its regulation by drugs and/or environmental factors
• The fluorophore is readily excited with the 488 nm argon-ion laser and fluorescence emission can be collected using a standard 530/30 nm BP filter on most flow cytometers, and thus is easily multiplexable with reagents used on other lasers
• Rapid and reproducible Wash steps and quencher dyes are not needed, since the pHrodo™ Green dye is non-fluorescent outside the cell, making the staining protocol simple and fast
• The elimination of wash steps and quencher dyes also improves assay reproducibility, particularly in plate reader-based assays
• Use with bacteria Analysis of phagocytosis by Gram-positive or Gram-negative bacteria is easily accomplished using the ready-made pHrodo™ Green BioParticles™ conjugates
• Use ready-made pHrodo™ Green BioParticles™ conjugates for convenient analysis of phagocytosis of Gram-positive or Gram-negative bacteria, or the amine-reactive pHrodo™ Green STP ester for labeling microorganisms or proteins of your choice
• pHrodo™ Indicators for Phagocytosis The pHrodo™ Green S
• aureus BioParticles™ Phagocytosis Kit is part of a larger product offering of reagents for measurement of phagocytosis
• Scientists can use a variety of pHrodo™ reagents in plate readers, fluorescence microscopy imaging and flow cytometry
• Additional information about pHrodo™ Green products, pHrodo™ Red products, and other pHrodo™ indicators for phagocytosis .