Invitrogen™ pHrodo™ Red Phagocytosis Particle Labeling Kit for Flow Cytometry

Description

• Specifically detect phagocytosis and endocytosis with pH-sensitive fluorogenic dye - discriminate endcytosed from adherent and extracellular particles Reduced signal variability and improved timing in sensitive experiments - no need for wash steps or quencher dye Bright red fluorescence - conveniently multiplex with green dyes such as GFP, Fluo-4, or calcein The pHrodo™ Red dye gives faster and more accurate results than any other phagocytosis assay The new Molecular Probes™ proprietary pH-sensitive rhodamine-based pHrodo™ Red dye is non-fluorescent at neutral pH, but turns bright red upon acidification
• Because it is both fluorogenic and pH-sensitive, the pHrodo™ Red dye can be used as a specific sensor of phagocytic events; acidification of the phagosome following phagocytosis is marked by red fluorescence
• It is therefore an ideal tool with which to study phagocytosis and its regulation by drugs and/or environmental factors
• Rapid and reproducible Wash steps and quencher dyes are not needed since the pHrodo™ Red dye is non-fluorescent outside the cell, making the staining protocol simple and fast
• The elimination of wash steps and quencher dyes also improves assay reproducibility, particularly in plate reader-based assays
• Flexible applications Use ready-made pHrodo™ Red BioParticles™ conjugates for convenient analysis of phagocytosis of Gram-positive or Gram-negative bacteria, or the amine-reactive pHrodo™ Red SE form for labeling microorganisms or proteins of your choice
• Compatible with multiple platforms pHrodo™ Red dye conjugates can be used in plate readers, fluorescence microscopy imaging, and flow cytometry applications
• The pHrodo™ Red Phagocytosis Particle Labeling Kit and the pHrodo™ Red E
• coli BioParticles™ Phagocytosis Kit for flow cytometry were designed for rapid and convenient measurements of phagocytic activity in whole blood samples by flow cytometry
• The kits include all the reagents required for assessing particle ingestion and red blood cell lysis
• The Labeling Kit also contains the reagents required for labeling microorganisms with the pHrodo™ Red dye
• Sufficient reagents are provided in both kits for approximately 100 assays
• The optimal absorption and fluorescence emission maxima of the pHrodo™ Red BioParticles™ conjugate is approximately 560nm and 585nm, respectively
• However, the fluorophore is readily excited with the 488nm argon-ion laser installed on most flow cytometers.