Thermo Scientific™ GeneBLAzer™ T-REx™-G2A-NFAT-blaFreeStyle™ 293F Cells
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1749-Each-of-1 | In Stock | ₹ 9,41,620.00 |
K1749 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 9,41,620.00
GST (18%): ₹ 1,69,491.60
Total Price: ₹ 11,11,111.60
Accession Number
NM_013345
Cell State
Dividing Cells
Detection Method
Fluorescence
Gene Symbol
G2A
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Cell Line
FreeStyle 293-F Cells
Druggable Target
GPCR
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ TREx™-G2A-NFAT-blaFreestyle293F cells• GeneBLAzer™ TREx™-G2A-NFAT-blaFreestyle293F Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ TREx™-G2A-NFAT-blaFreestyle293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application)
FRET
Population Status
Single Cell Clone Origin
Quantity
2 x 106Cells
Target Entry
GPR132, G2A
System Type
GeneBLAzer™
Related Products
Description
- GeneBLAzer™ T-REx™ G2A-NFAT- bla Freestyle HEK 293F cells contain the human GPR132 (G2A), (Accession No
- NP_037477) stably integrated into the CellSensor™ NFAT- bla Freestyle HEK 293F cell line
- CellSensor™ NFAT- bla Freestyle HEK 293F cells (Cat
- no
- K1725) contain a beta-lactamase reporter gene under control of the NFAT Response Element
- The GeneBLAzer™ T-REx™ G2A-NFAT- bla Freestyle HEK 293F cells are functionally validated for Z' and EC 50 concentrations of dDAVP
- In addition, GeneBLAzer™ GeneBLAzer™ T-REx™ G2A-NFAT- bla Freestyle HEK 293F cells have been tested for assay performance under variable conditions
- These data are found in the Validation & Assay Performance Summary
- G2A is an orphan GPCR
- Upon overexpession G2A is constitutively active
- This is an assay for the orphan GPCR based upon constitutive activity
- To use the assay to detect inverse agonists, high amounts of doxycycline should be added
- To use the assay to detect agonists, low amounts of doxycycline should be added
- For Academic pricing please login or contact us at discoverysciences@invitrogen.com.