V23201

Invitrogen™ Chromatin Condensation & Membrane Permeability Dead Cell Apoptosis Kit with Hoechst 33342, YO-PRO™-1, and PI dyes, for flow cytometry

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 V23201-Each-of-1 In Stock ₹ 34,977.00

V23201 - Each of 1

₹ 34,977.00

In Stock

Quantity

1

Base Price: ₹ 34,977.00

GST (18%): ₹ 6,295.86

Total Price: ₹ 41,272.86

Quantity

1 kit

Product Type

Dead Cell Apoptosis Kit

Flow Cytometer Laser Lines

488 nm

No. of Reactions

200 Reactions

Product Line

Vybrant™, YO-PRO™

Conjugate

Hoechst 33342, Propidium Iodide, YO-PRO-1

Form

Solution

Format

Kit

Content And Storage

Contains 1 vial of Hoechst 33342, 1 vial of YO-PRO™-1, and propidium iodide.Store in refrigerator (2–8°C) and protect from light.

Excitation/Emission

YO-PRO™-1: 491/509 cm, Hoechst 33342: 350/461 cm, PI: 535/617 cm

Shipping Condition

Room Temperature

For Use With (Application)

Flow Cytometry

For Use With (Equipment)

Flow Cytometer

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Description

  • This product detects apoptotic cells with changes in nuclear chromatin condensation and plasma membrane permeability, using three nucleic acid stains: UV-excitable Hoechst 33342, green fluorescent YO-PRO™ dye, and propidium iodide
  • The YO-PRO™ dye can enter apoptotic cells, whereas red-fluorescent propidium iodide (PI) cannot
  • Thus after staining with YO-PRO™-1 dye and PI, apoptotic cells show green fluorescence, and dead cells show primarily red fluorescence and some green fluorescence
  • Blue fluorescent Hoechst 33342 brightly stains the condensed chromatin of apoptotic cells and more dimly stains the normal chromatin of live cells
  • The staining pattern resulting from the simultaneous use of these three dyes makes it possible to distinguish normal, apoptotic and dead cell populations by flow cytometry or fluorescence microscopy.