Invitrogen™ EVOS™ Objective Starter Kit for Fluorescence/Brightfield/Phase Contrast

Description

• This fluorite objective bundle includes three objectives and is excellent for general brightfield (transmitted light), phase-contrast, and fluorescence applications
• The objectives included in this bundle are 4X, 10X, and 20X fluorite long-working-distance (LWD) phase-contrast objectives
• LWD objectives are optimized for the imaging of slides, cell culture dishes/flasks, and microtiter plates
• Phase-contrast imaging improves the image quality for hard-to-see, translucent specimens (such as cells)
• All EVOS objectives offer outstanding optical performance from visible light to near-infrared light
• Additional characteristics of this EVOS objective bundle: EVOS 4X Objective, fluorite (Cat
• No
• AMEP4980) Magnification: 4X Numerical aperture: 0.13 Working distance: 10.58 mm EVOS 10X Objective, fluorite (Cat
• No
• AMEP4981) Magnification: 10X Numerical aperture: 0.30 Working distance: 7.13 mm EVOS 20X Objective, fluorite (Cat
• No
• AMEP4982) Magnification: 20X Numerical aperture: 0.45 Working distance: 6.12 mm Image quality Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation
• Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light
• This performance results from years of lens manufacture perfection
• EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements
• Objective classes Achromat objectives are excellent for general applications, with standard correction of color and focus
• Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging
• The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives
• Fluorite objectives are well-suited for fluorescence and demanding transmitted light applications, where the higher contrast make them well-suited for color imaging
• Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated
• They are recommended for the most demanding applications, particularly at magnifications of 60x and above
• Apochromatic objectives are the a great choice for the capture of color images in white light
• Brightfield contrast versus phase contrast objectives Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light
• A higher density area in a sample will absorb more light, thus increasing contrast in those areas
• Phase contrast objectives are most useful for hard to see, translucent specimens
• This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast)
• Long working distance versus coverslip-corrected objectives Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9–1.5 mm
• This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc
• Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm)
• These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.