Description

• General description: Tris is an established basimetric standard and buffer used in biochemistry and molecular biology.[1] It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc.[2] It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.[3]
• Application: Trizma® base was used as buffer for the following studies:Electrophoretic transfer for the specific identification of isozymes of starch debranching enzyme, α-amylase and 9-amylase.[4]Electrophoretic separation of lipoproteins in polyacrylamide gels.[5]Preparation of TRIS buffer having pH 8.[6]It may be used to compose DN buffer for DNA nick-end labeling of tissue sections.[7]
• Other Notes: The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
• Legal Information: Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany