Description

• General description: DMT-2′O-Methyl-rU Phosphoramidite belongs to the class of 2′O-Methyl RNA Phosphoramidites.
• Application: 2′O-Methyl RNA nucleoside including DMT-2′O-Methyl-rU Phosphoramidite can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.
• Features and Benefits: Its key features include: High yield of crude oligonucleotides Compatible with DNA synthesis Can be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotides Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA (concentrated ammonia/ 40% aqueous methylamine I/I, v/v) for 10 minutes at 65 °C Purification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as no special precautions are required to provide protection against nucleolytic degradation Synthesis of 2′O-Methyl RNA oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 6 minutes compared to 90 seconds for DNA monomers) 2′O-Methyl RNA phosphoramidites are also available with fast deprotection chemistry
• Other Notes: The unique combination of properties of 2′O-Methyl RNA had found widespread use in the fields of: Diagnostic probes Aptamer and ribozyme development Mixed 2′O-Methyl-RNA/DNA antisense molecules
• Legal Information: OligoPilot is a registered trademark of Cytiva