809705
5-DBCO-PEG4-dUTP, 10mM Aqueous Solution
Manufacturer: Sigma Aldrich
Synonym(S): 5-DBCO-PEG4-dUTP
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 50 μL | 809705-50-μL | In Stock | ₹ 27,235.70 |
809705 - 50 μL
In Stock
Quantity
1
Base Price: ₹ 27,235.70
GST (18%): ₹ 4,902.426
Total Price: ₹ 32,138.126
Assay
>95% (HPLC)
Quality Level
200
form
solution
reaction suitability
reaction type: click chemistry
solubility
Tris buffer: 100 mM (pH 7.5)
shipped in
wet ice
storage temp.
−20°C
SMILES string
O=C1NC(N([C@H]2C[C@@H]([C@@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)O2)O)C=C1/C=C\CNC(CCOCCOCCOCCOCCNC(CCC(N3CC4=CC=CC=C4C#CC5=CC=CC=C53)=O)=O)=O)=O
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Description
- Application: 5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR with Taq polymerase. It is enzymatically incorporated into DNA/cDNA as a substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can be labeled with azide-labeled molecules using copper-free click chemistry. This enables experiments such as introduction of a biotin group for purification tasks, introduction of a fluorescent group for detection, or crosslinking the DNA to azide-functionalized biomolecules such as proteins.
SAFETY INFORMATION
WGK
WGK 2